ABSTRACTS 2016 #1                                  
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THE EFFECT OF POTASSIUM AND GIBBERELLIC ACID COMBINATION ON GROWTH
AND DEVELOPMENT OF Cucumis sativusL. cv.Sevenstar

Sevenstar Priyanka PAL, Kuldeep YADAV, Krishan KUMAR, Narender SINGH

Abstract. Cucumis sativusL. (Family: cucurbitaceae) is one of the most important consuming annual herbaceous vegetables rich in calcium, magnesium, thiamine, niacin and vitamin C. A study was carried out to evaluate the potential of exogenously applied potassium (1.0, 2.5 and 5.0 g·l-1) and gibberellic acid (0.005, 0.01 and 0.015 g·l-1) in combinations on vegetative growth and development of F1 hybrid of Cucumis sativusL., cv. Sevenstar. Data were recorded for vine length, fresh weight, dry weight, branch number, leaves number, absolute growth rate, relative growth rate, biomass duration, chlorophyll, carotenoids, calcium and magnesium contents of leaves. Application of combinations of potassium and gibberellic acid also increased the yield quantity (% fruit set, fresh and dry weight). The treatment containing potassium at 2.5 g·l-1 and gibberellic acid at 0.015 g·l-1 was best for growth of cv. Sevenstar. Therefore, it can be concluded that foliar application of potassium and gibberellic acid may be effective strategy for maximizing the growth and development of parthenocarpic cucumber.

Keywords: Cucumis sativus; gibberellic acid; growth; parthenocarpic; potassium

Abbreviations: GA3- Gibberellic acid; K – Potassium; AGR - Absolute growth rate;
RGR - Relative growth rate; BMD -biomass duratio


BIOSYNTHETIC POTENTIAL OF Saccharomyces YEASTS AT THE TREATMENT
WITH EXTREMELY HIGH FREQUENCY MILLIMETER WAVES


Natalia CHISELIŢA, Agafia USATÎI, Ludmila BEJENARU, Elena TOFAN, Nadejda EFREMOVA

Abstract. Experiment results of the study of extremely high frequency millimeter waves (EHF) of 60.12; 53.33 and 42.19 GHz effect on biomass accumulation and biosynthetic activity of Saccharomyces cerevisiae CNMN-Y-20 and Saccharomyces cerevisiae CNMN-Y-18 yeast strains – polysaccharides producers deposited in National Collection of Nonpathogenic Microorganims of Institute of Microbiology and Biotechnology of Academy of Sciences of Moldova have been revealed. Duration of treatment of yeast strains in carried out study constituted 10, 20, 30 minutes. Research has demonstrated the efficiency of extremely high frequency electromagnetic radiation for stimulation of biosynthetic processes at yeasts manifested in the increase of bioactive substances content while the stimulation or maintaining at the same level of biomass production.
The irradiation of yeast strains with millimetric waves contributed to the increasing of carbohydrates with 29-43.5%, ß-glucans with 17.4-25.7%, mannans with 11.2-21% and protein content with 26.9-49.9 % as well as to the increasing of catalase activity with 34.5-69.2% compared to the control in dependence on strain, used frequency and duration of irradiation.

Keywords: Saccharomyces cerevisiae,biologically active substances, extremely high frequency millimeter waves.


CONTRIBUTIONS TO THE PHYTOSOCIOLOGICAL STUDY OF THE SALICI PURPUREAE-MYRICARIETUM MOOR 1958 ASSOCIATION IN THE ORĂŞTIE RIVER BASIN
CENTRAL-WESTERN ROMANIA)

Valeriu-Ioan VINŢAN

Abstract. In the current paper we present a phytosociological study of the phytocoenoses of the association Salici purpureaeMyricarietumMoor 1958 identified on the bank gravels of upper Orăştie river basin, situated in the central-western part of Romania. The characterisation of the association under analysis as well as the presentation of the phytosociological table have been done by selecting the most representative relevés performed in the undergrowths of Myricaria germanicain the upper Orăştie river basin. The phytocoenoses of this particular association were analysed in terms of physiognomy and floristic composition, life forms spectrum, floristic lements, and ecological indices. The Myricaria germanica underbrushes represent a highly community-significant natural habitat of great conservational value, whose preservation calls for the delineation of certain off limit areas.

Keywords: phytocoenoses, association, relevés, floristic elements, life forms, ecological indices, Myricaria germanica.


ECOLOGICAL RESEARCH ON THE DYNAMICS OF ARTHROPODS FROM GRASSLANDS
ON THE OUTSKIRTS OF "DUMBRAVA SIBIULUI" FOREST (SIBIU, ROMANIA) IN THE PERIOD 2012 - 2014

Cristina STANCĂ-MOISE, Maria TĂNASE

Abstract. At international and European level, great emphasis is laid upon preserving various ecosystems biodiversity; the current researches make Romania meet these goals. Knowing the composition of the fauna and of the interrelations between the structural parts of the examined biocenotic, a grassland on the outskirts of Sibiu city located in central Romania, is of particular importance for making ecological studies. For assessment of its current condition, we have been conducting a comprehensive research over three years: 2012, 2013, 2014, during the months of July, August, September, examining the dynamics of arthropods, intended to ensure their inventorying and monitoring, with a total of 49.422 specimens collected during the said three years. The biocenotic structure of arthropods from grassland is superficially displayed in the lists of the collected species. The quantitative relationships between the number of specimens and the reported number of species can be achieved by using ecological indicators used in analyzing the data obtained. This paper is a starting point to further research in this direction.

Keywords: arthropods, diversity, grassland, zoogeography, dynamics.

 

IN VITRO COMBINED ANTIBACTERIAL EFFECT OF TURMERIC (Curcuma longa)
AND GINGER (
Zingiber officinale) ON SOME PATHOGENIC ORGANISMS
 

Kelechi Mary UKAEGBU-OBI, Afoma Chinwe IFEDIORA, Nonye Helena IFEDIORA, Blessing CHUKWU 

Abstract. The antibacterial effects in vitroof crude ethanol and aqueous extracts of Turmeric (Curcuma longa), Ginger (Zingiber officinale)and Turmeric and Ginger combined were assayed against E. coli, S. aureus, P. aeruginosaand S. enterica Type typhi. The antibacterial activity was determined using the well diffusion method. Turmeric (C. longa) showed least antibacterial activity against the test organisms. The combined extracts of C. longa and Z. officinaleexhibited higher antibacterial activity than C. longaalone. Ginger (Z. officinale)extracts were most effective. Ethanolic extracts were found to be more potent than the aqueous extracts. The plants’ extracts were least active against S. enterica Type typhi and most active against P. aeruginosa. Chloramphenicol which served as the control had higher activity than the spices singly and combined. The Minimum Inhibitory Concentration (MIC) of the ethanolic extracts of plants was 50-150 mg/ml for C. longa,50-100 mg/ml Z. officinale and 50-150 mg/ml in synergy. Results of this kind exhibit an interesting promise of designing a potentially active antibacterial combined agents of plant origin. 

Keywords: Combined; Antibacterial effect; Turmeric; Ginger; Chloramphenicol; Pathogenic organisms. 

CURRENT POLITICAL FRAMEWORK REGARDING CONTROL MEASURES ON POTATO CYST NEMATODES IN ROMANIA 

Maria-Mihaela ANTOFIE

Abstract The scope of this article is to reveal major gaps in the research needs for the support of implementing the phytosanitary quarantine monitoring plan for Globodera rostochiensisand G. pallidaknown as potato cysts nematodes (PCN) in line with the new Directive 2007/33/EC. Romania is currently cultivating potato on 1.8 % of the arable land and according to this plan it needs to analyse over 2,500.00 soil samples at least. The financial needs in implementing this Directive are high, only the costs associated with soil sampling being estimated to be at least 2.4 mil Euros for a period of 12 years (i.e. between 2007 and 2019). The budget may rise up to 5.6 mil Euros in case of failing the implementation of the current monitoring plan by 2019. Additionally controlling measures have been investigated and this study support the idea that Romania needs to further develop research for the 19 counties currently cultivating potatoes for weed control, feral and livestock movements in order to ensure the successful implementation of the current monitoring plan. The biological control of PCNs in direct connection with potential host plants or animal species that may influence their dissemination are research subjects of outmost importance. 

Keywords: Globodera rostochiensis, G. pallida, potato , phytosanitary quarantine, control measure, Romania.

CHANGES OF THE COMPOSITION OF THE BLOOD IN BROILER CHICKENS UNDER THE INFLUENCE OF BIOPRODUCTS BASED ON STREPTOMYCETES

Nicolae STARCIUC, Natalia OSADCI, Alexandr MANCIU, Maxim BYRSA, Svetlana BOORTSEVA

Abstract. Due to the shortage of feed, in particular a permanent deficit increasing protein feed with sufficient content of essential amino acids for feeding purposes are widely used microbial synthesis. The aim of our research was conducted to study the influence of drugs on the basis of Streptomyces fradiae19 to change the composition of blood of broiler chickens during their growth. For obtaining biomass and complex of exometabolites, the strain was grown on complex medium R, comprising as main sources of carbon and nitrogen – corn flour, starch and a number of mineral salts. According to results the quantity of total proteins and albumins increase essential by administration in drink water of cultural liquid in concentration 1 ml / l compare with control by 32.6% and 29.2% respectively. The cholesterol decrease significantly only in first two groups (control and with use of biomass), but in the third is practically unchanged (cultural liquid). Best results according to quantity of triglycerides were obtained by using of cultural liquid, too (increased by 2.6 times more). Compare with control and group where was administrated in feed ratio 0.1% of biomass, after adding of cultural liquid the quantity of monocytes is more by ~30%; but according to values of lymphocytes, they are preserved on the same level after use of biomass in feed and significantly decrease in groups of control and where is use cultural liquid. In values of leukocytes, for all three groups there are not significant changes. Obtained results show the perspective of the direction of using products of microbial nature. 

Keywords: Streptomyces fradiae; broiler chickens; drugs; biological products; proteins; albumen; cholesterol; triglyceride; lymphocytes; monocytes; leukocytes; non-segmented neutrophils.


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