Abstract. From the rhizosphere of tomatoes were isolated bacterial cultures demonstrating antifungal properties. There was carried out a series of tests after which the bacterium was identified as Bacillus subtilis, they were given worker numbers - S2 and S4. Bacteria have been reported to grow as colonies, stroke or continuous lawn on the surface of solid cultivation media, depending on the mode of spread (inoculation). The cultural morphology features of bacteria growth were studied on the conventional media: MPA, PDA, M9. Both cultures gave good growth on the represented media, which differ in specific features typical for this isolate. Bacillus subtilisS2 gave the certain well-defined colonies only on medium IPA, but on the other two media the colonies blurred, blurring the boundary of the colony. Bacillus subtilisS4 grew as sharply defined colonies in all of the three media. In this paper, we describe the differences in bacterial growth when inoculated by streak. Bacterial growth in the liquid media also had characteristic features: the presence of sediment and biofilm, pigmentation of cultivation media.

Abstract. Was studied the antimicrobial activity (antibacterial, antifungal) of the strain Streptomyces fradiae 19 isolated from chernozem soil of the Central Part of the Republic of Moldova. Study of the properties was carried out by using the test-cultures of opportunistic pathogens (E. coli, P. aeruginosa, E. faecalis, S. aureus, C. albicans) microorganisms and plant pathogenic bacteria (A. tumefaciens, C. michiganensis, X. campestris) and fungi (А. alternata, А. niger, B. cinerea, F. oxysporum, F. solani). Metabolites of studied strain showed the highest activity against the following test-microorganisms: S. aureus, C. albicans, growth inhibition zones ranged from 18.0 mm till complete inhibition in dependence of growing medium, but against phytopathogenic bacterium X. campestris, the diameter of the growth inhibition zones were from 24.5 till 32.0 mm.

Abstract. Studies on the genetic variation and structure of plant population are important for evaluation of dynamics population and conservation management. We studied the population genetic variation of Onobrychis viciifoliain five ecologically different regions in East-Azerbaijan, Iran using Inter-Simple Sequence Repeats (ISSR) markers. Populations' genetic diversity were estimated based on Nei’ and Shannon's indices using polymorphic ISSRs loci. The relationship of populations' geographical and genetic distances and also population altitude and ISSRs variations were investigated. Similarity among populations was assessed by UPGMA clustering analysis. The results showed that polymorphic ISSRs loci varied from 38.75% to 61.25% among populations. Within-population Nei's diversity ranged from 0.1182 to 0.1790. Partitioning of total ISSRs diversity by AMOVA revealed that 67.45% diversity allocated to within- and 32.55% diversity to among-populations, indicating that O. viciifoliais predominantly an outcrossing species. This study showed that population geographical distances was not correlated with Nei distances (N=10, P vale ≥0.56) suggesting lack of gene flow among populations. Similarly, the altitude of populations had no impact on populations ISSRs variations (N=5,P value≥0.227). The populations of O. viciifoliagrowing under salinity stress had both higher and lower ISSRs variability. This cast doubt on Nich-Width Theory, which expects higher variations from stressful populations. Therefore, it can be concluded that populations' genetic patterns in O. viciifoliamight be affected by random change, rather than ecological selection. Comparison between the levels of ISSRs variations and those levels of RAPDs previous reported from the same populations showed that RAPDs detect significantly greater variation than ISSRs (N=5, P value ≤0.01, at P≤0.05 level).

Keywords: ecological selection, genetic diversity, genetic similarity, ISSRs, outcrossing, Onobrychis viciifolia, Sainfoin



Agafia USATII, Elena KIRITSA, Alina BESHLIU

Abstract. The paper provides new data about the effect of Fe₃O₄ nanoparticles with dimensions of 10 nm and 30 nm on bioproduction parameters of Rhodotorula gracilis CNMN-Y-30 strain. It has been established that size of nanoparticles and their concentrations did not change cells prolificacy and the content of cell biomass. It was found that the protein content was higher in yeast biomass up cultivation in the presence of iron oxide nanoparticles with dimensions of 30 nm, particularly at low concentrations of 0.5 and 1.0 mg / L, compared to the 10 nm size nanoparticles.

Keywords: Rhodotorula gracilis; nanoparticles; multiplication; carbohydrates; proteins.



Anca MIHALY COZMUTA, Leonard MIHALY COZMUTA, Anca PETER, Camelia NICULA

Abstract. Red bell pepper fruits were subjected to six weeks storage in the darkness, ultraviolet light (UV-C) and far red light (FRL), respectively at 8oC and 55% relative humidity. Fresh and stored fruits were sampled and analyzed in terms of wax specific amount and chemical composition, wax-cuticle wettability, barrier properties of cuticle against transpiration and weight loss. Wax biosynthesis occurred in both UV-C and FRL – exposed fruits and moreover in a higher hydrophobic ratio, results in an enhanced wax-cuticle compatibility and adhesion. On contrary, the cleavage of hydrophobic compounds in some more hydrophilic occurred into the wax of the darkness-stored peppers, increases the hydrophilic nature of wax, weakens the wax-cuticle adhesion and reduces the wax spreadability over the cuticle. As results, the barrier properties against water loss are debilitated to a larger extent in the darkness-exposed peppers and to a lesser extent in the FRL-exposed peppers. The water loss rate is positively correlated with the wax specific amount and its hydrophobic nature, the fruits exposed to the FRL displaying the lowest water loss rate followed by the fruits exposed to UV-C.

Keywords: far red light, UV-C, red bell peppers, wax specific amount and composition, weight loss



Maryam BARGHI, Hassan MONIRIFAR, Ahmad Razban HAGHIGHI, Ahmad GHANBARI, Seyed Abolghasem MOHAMMADI, Baratali SIASAR

Abstract. Alfalfa (Medicago sativa) is a major perennial forage legume. Random Amplified Polymorphic DNA markers were used to assess genetic diversity within and between populations collected from different area of East Azarbaijan province, Iran. A total of 135 and 109 fragments were amplified using 10 arbitrary primers in individual and bulk samples showing 97.04 and 100% polymorphism, respectively. Analysis of molecular variance (Amova) revealed higher genetic variation within population than between population compared with that of between populations. The result indicates that RAPD markers could provide useful information for a breeding program and the method could be useful for estimating genetic relatedness among the heterogeneous crop like alfalfa.

Keywords: alfalfa, genetic variation, RAPD, analysis of molecular variance



Cristina STANCĂ-MOISE

Abstract. Results of research work carried out in Sibiu (Romania) on the biology, ecology and ethology of Neoglocianus maculaalba (Herbst, 1795) (Coleoptera, Curculionidae) are reported. The adults hibernate at 10-40 cm deep in the soil where from they came out in the first ten days of June in the year 2014 when the average temperature was 14 °C, the maximum number of individuals that got out was achieved at 16 °C. Copulations and laying of eggs begun at 18 °C. A maximum attack was recorded when 75% of the plants were in blossom with very young capsules. Under laboratory conditions the rate per day of egg laying by a female was between one and 50 eggs, the maximum number of eggs laid was 114 in 10 days. The larva growth period was of 13-20 days at 26-27 °C and 65-70% moisture. The larvae hatched out from the capsules and the pupa period began particularly around 6-7 a.m. and 8-9 p.m., the number of larvae getting out from the capsules to become pupae ranged from 1 to 18 p.m. Based on the observations on insect biology, ecology and ethology, treatment is recommended at the moment when 70% of the poppy plants are budding and when almost all insects are coming out from soil.

Keywords: poppy; biology; ecology; ethology; Neoglocianus maculaalba.



Nicolae COJOCARU, Carmen Liliana BĂDĂRĂU, Florentina STROE

Abstract. The objective of this research was to study if the frozen material could be used to obtain a PVY suspension with a proper concentration for antisera preparation. The virus extraction and purification was achieved from frozen leaves and fresh tobacco leaves (cv. ‘Samsun’ and ‘White Burley’) mechanically inoculated with a PVYN infected material (cv. ‘Record’ secondary infection with this pathogen). The experiment followed a schema used in other virology laboratories with some modifications particulary quantitative. Results of this preliminary studies indicated that frozen PVYN infected material is not advisible for the preparation of a virus suspension solution to be used for antisera production.

Keywords: potato virus Y, necrotic strains, purified preparation
Abbreviations: PVY = potato virus Y; PVYN =PVY necrotic strains