ROLE OF CHELATED ZINC (ZN-HEDTA) FOLIAR SPRAY IN REGULATION SALT STRESS IN MAIZE PLANTS
Zeinab Abd El Rahman SALAMA, Mohamed Mostafa EL FOULY, Alaa Abd Allah GAAFAR
Abstract. Salinity is an important constraining factor in the way of crop production around the world. Plant nutrition management could alleviate the harmful effects of salinity. In order to evaluate the interaction effects of Zinc availability and salinity, an experiment was conducted using Zinc HEDTA (20 µmol) and 100mmol of NaCl salinity with two cultivars (single cross -10 and single cross-162 ) of maize plants. Results implied that chlorophyll contents and carbonic anhydrase (CAA) which destructively affected by salinity were improved by Zn foliar spray. According to the results, antioxidant activity of peroxidase (POD) increased by the combination of salinity and Zn to a great extent compared to sole salinity. Our results indicated that the accumulation of 22 KD M.W of proteins was in response to salinity stress. The results showed that Zn (II)HEDTA induced the synthesis of new protein bands. According to the results obtained, it seems that Zn (II)HEDTA can be used as novel chelates to supply Zn and to increase tolerance to salt-stress of maize in salinized hydroponic nutrient solution.
Keywords:Salinity; Zn-HEDTA; Photosynthesis; carbonic anhydrase; peroxidase; maize; cultivars.
Ana VALUTA , Liliana CEPOI , Ludmila RUDI , Ion BULHAC , Paulina BOUROSH , Olga BOLOGA
Abstract. The article deals with iron(III) coordination compounds with Schiff bases as ligandsand their impact on phycobiliprotein accumulation by cyanobacterium Nostoc linckia. Stimulatory effect depends on the applied dose and in case of three compounds, the concentration 20 mg/L was determined as one with moderate intensity. Lower concentrations resulted in an increase of the phycobiliprotein synthesis. There was found a significant positive correlation between phycobiliprotein content and ABTS (2.2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay values displayed by aqueous extracts from Nostoc linckia biomass cultivated in nutrient medium with these coordination compounds. Hence, it is possible to modify the antioxidant activity of Nostocbiomass by applying low concentrations of chemical stimuli.
Keywords: phycobiliproteins, cyanobacteria, Nostoc linckia, antioxidant activity, ABTS assay, correlation
THE FREQUENCY OF Q-FEVER IN FARM ANIMALS IN WESTERN MACEDONIA
Ismije SAITI, Kristaq BËRXHOLI
Abstract. The Q fever is a zoonotic disease, caused from Coxiella burnetii, an obligate intracellular, pleomorphic coccobacillus possessing a prototypic gram-negative bacterial cell wall structure. The aim of study was to examine the frequency of Q fever in farm animals (sheep, goats and cows) and the frequency variation according to species in five regions in Western Macedonia (Tetovo, Gostivar, Kicevo, Debar and Struga). The data from this study indicate for the presence of Q fever in these areas. A total of 1,120 farm animals were examined, of which 178 serums resulted positive, with a scale of 15.89% positivity. The infection is quite widespread in all of the five regions and varies from 8.0% in Gostivar up to 27.71% in Kicevo. As regards the variation of frequencies based on species, the infection is widespread in all three species and in every region under surveillance; in sheep, it varies from 14.65% in Gostivar to 42.04% in Kicevo; in goats, from 2.89% in Gostivar to 14.28% in Debar; and in cows it varies from 4.42% in Gostivar to 12.96% in Kicevo. The serums were conserved in -30 °C and as a serological test ELISA from ID vet Monpelie France was used, which was carried out based on its relevant protocol using purified antigen of C. burnetii.
Keywords: Q-fever, Elisa test, Coxiella burnetii, relative frequency, farm animals.
THE EFFECT OF FRUIT LOAD ON POD AND SEED CHARACTERISTICS OF OKRA (Abelmoschus esculentusL.)
Ghadir MOHAMMADI, Ebrahim KHAH, Spyridon Alexandrou PETROPOULOS, Dimosthenis CHACHALIS
Abstract. A study to determine the effects of fruit load on the pod and seed characteristics of okra was carried out. Four cultivars of okra (‘Boyiatiou’, ‘Veloudo’, ‘Clemson’ and ‘Pylaias’), very common in Greece and the Mediterranean Basin, were cultivated under field conditions and three levels of fruit loading [L1, L2,and L3(10, 15 and all fruits plant -1 respectively)] were applied on plants. . Leaving more fruit on the plant (L3) caused lower seed germination in cv. ‘Boyiatiou’ and ‘Veloudo’, where in cv. ‘Pylaias’ and ‘Clemson’ the opposite effect was observed. Highest germination was recorded for plants with the lowest fruit load (L1). The most seedhardness and least seed germination was observed in cv. ‘Boyiatiou’, but storage of seeds for 18 months increased germination by up to 16.2%. Flower induction, fruit set and fruit dimensions were unaffected by fruit load, irrespective of cultivar. Seed number per fruit was not affected by fruit load, but seed size (mean 100 seed weight) tended to decrease with fruit load in cv. ‘Boyiatiou’ and ‘’Pylaias’. There was also no consistent effect of fruit load on plant height. In conclussion, pod load is not considered to affect okra seed quality and especially for crops intended for seed production growers should not apply pod thinning since no effect in seed quality was observed.
Keywords: Abelmoschus esculentusL.; fruit load; okra; pod set; seedhardness.
INFORMATION ON THE MACROLEPIDOPTERA FAUNA OF “DUMBRAVA SIBIULUI” OAK FOREST (SIBIU, ROMANIA)
Cristina STANCĂ-MOISE
Abstract. In order to establish systematic, ecological and ethological studies, it is extremely important to know the composition of faunal species of Lepidoptera collected in the past and present from "Dumbrava Sibiului" Forest located on the outskirts of Sibiu in central Romania. To assess the current state of Lepidoptera species, we conducted a complex research which was carried out for over 13 years, based on our own samples collected: from March to November, but also on the data resulted from studying the species existing in the collections of the Natural History Museum of Sibiu. After the research we have identified a number of 243 species belonging to 14 families collected over time from "Dumbrava Sibiului" Forest.
Keywords: Macrolepidoptera, Heterocera, Forest "Dumbrava Sibiului", faunistics, ecology, zoogeography.
HISTOPATHOLOGICAL ANALYSES OF THE LIVER AND GONADS OF VARDAR RIVER FISH Barbus peloponnesius
Nexhbedin BEADINI, Sheqibe BEADINI, Gazmend ISENI, Hesat ALIU, Xhezair ABDIJA, Avdi NAZIFI, Albulena BEADINI, Ibadete DENJALLI
Abstract. The Barbus pelopnnesiusis a typical benthophagous fish, feeding on the zoobenthos and plant components and thanks to its sensitivity to the changes in surrounding area is an ideal tool for indication of the health of aquatic ecosystems. The aim of this study was histopathological analysis of the liver and gonad tissue of Barbus peloponnesiusfish of Vardar River. Liver and gonad of 25 fish individuals collected from two research stations, at the exit point of Veles city during 2013, were excited and proccesed for standart histopathological analysis. Microscopically analysis revealed pathological changes in the liver and gonad tissue including hypertrophy of epithelial cell nuclei in bile ducts and proliferation of their epithelium, presence of hemosiderin, hemorrhage in the hepatocellular parenchyma, bacterial and verminous infection, as well as macrophagous aggregation. Estimation of water quality from two points of vardar River near Veles on the basis of physic-chemical and microbiological indicate that waste water have negative impact to the natural fish population.
Keywords: fish; biomarkers; Barbel; histopathological; lesions; liver; gonads; aquatic ecosystems.
