Muhammad ISMAIL, Salma JAVED, Zahra BATOOL, Kiran ZAHRA, Iffat DIN, Salma SHAHEEN, Ejaz HUSSAIN, Yupei YANG, Wei WANG

Abstract: Medicinal plants play a vital role in human life. The plant-based botanicals are considered to be safer, selective, and effective to conventional and synthetic chemicals used for the same purposes. There are huge number of bioactive plants around the globe which needs to be explored for their potential as herbal alternatives to traditional chemicals. In the present study, the methanolic extracts of Anaphalis nepalensis (AN), Anaphalis virgata (AV), Artemisia foetida (AF), and Anthemis cotula (AC) were collected from Gilgit-Baltistan. The main plant extracts were further fractionated into n-hexanes (H), dichloromethane (D) and aqueous (A) fractions. All these fractions were analyzed for their nematocidal, insecticidal, anti-inflammatory, and anti-cancer activities. All three fractions of AC, AV, and AF showed excellent nematocidal activity against Meloidogyne incognita (90, 94, 95%, respectively) at conc. of 1% after 72 h of the treatment. Similarly, AV-D and AC-D showed excellent anti-inflammatory response with IC50 values of 20 and 32 through inhibition of ROS. The cytotoxic activities of AC-H, AC-D, and AC-A against Hela tumor cell lines was found active with IC50 values of 22.7, 12.7, and 7.6, respectively.

Key words: Nematocidal; insecticidal; Meloidogyne incognita; green pesticides; anti-cancer; anti-inflammatory.




Mohammed SOUDDI, Bahae-Ddine BENDI-DJELLOUL-GHEZLAOUI

Abstract. The aim of this study is to propose a bioclimatic classification for Tlemcen (NW Algeria) based on the recently developed Global Bioclimatic Classification System (GBCS). The GBCS classification is widely used in vegetation science and landscape ecology. Temperature and precipitation data from 9 weather stations were employed for the bioclimatic classification. The results show that, two macrobioclimates, two thermotypic horizons were identified. The Mediterranean macrobioclimate is the dominant pattern, including a wide range of bioclimates such as the Mediterranean pluviseasonal-continental and Mediterranean xeric continental. Temperate macrobioclimate has only one bioclimate i.e. Temperate Continental. The bioclimatic classification proposed in this study is useful for assessing the relationship between climate and the distribution of species.

Key words: bioclimatic classification; Mediterranean region; Tlemcen; vegetation; Algeria.




Svetlana BOORTSEVA, Maxim BYRSA, Irina SHIBAEVA, Alexandru SHIBAEV, Nicolai CEBOTARU, Svetlana CODREANU

Abstract. The paper deals with the research results on changes in antimicrobial activity of strain Streptomyces canosus CNMNAc-02 and its 3 variants (obtained using γ irradiation, combined (γ and UV) mutagenesis, low-frequency and low intensity magnetic field) after long-term storage (20 years) by subculturing. Antimicrobial activity was determined by the disk diffusion method, as test cultures were chosen opportunistic pathogenic bacteria and fungi which cause crop diseases. Analysis of the results obtained allow us to recommend oat agar medium for long-term storage of the original S. canosus CNMN-Ac-02 strain with antibacterial activity against phytopathogenic bacteria, and Czapek medium with glucose to preserve antifungal activity. For S. canosus CNMN-Ac-03, the antifungal activity is preserved on Gause medium, and for S. canosus CNMN-Ac-04 - on Czapek medium. For variant treated with magnetic field, higher antibacterial activity was registered against Clavibacter michiganensis 13a on Gause medium, by cca. 11.0 % more than original strain. Also it was noted, that strains synthesize several antibiotics, because of ability to inhibit develop both of phytopathogenic fungi and bacteria.

Key words: Streptomyces canosus; antimicrobial activity; subculturing; long-term storage; synthetic media; organic media.




Budiyati ICHWAN, Rujito Agus SUWIGNYO, Susilawati SUSILAWATI, Eliyanti ELIYANTI, Zulkarnain ZULKARNAIN

Abstract. This study aimed at examining the effect of fertilizer and water application through leaves to increase the growth and yield of chili pepper under drought stress conditions. The study was arranged in a split plot design with three replications. The main plot was plant cultivar (Kastilo, Lado and Romario), while the subplot was the application of fertilizer through leaves (no fertilizer and no water, application of 5 mL·L-1 foliar fertilizer, and water only). The results showed that different cultivars of chili peppers gave a positive response to the application of water and fertilizer through the leaves under drought condition (50% of field capacity), and the application of 5 mL·L-1 foliar fertilizer produced better growth and yields. Application of liquid fertilizer through leaves increased the growth of chili pepper by 57.43% - 82.49% and the production by 81.59% - 94.09%. The number of branches and fruit weight were strongly affected by the application of liquid fertilizer through leaves. The cultivar that were most responsive to the application of foliar fertilizer under drought stress conditions was Lado, followed by Kastilo and Romario. The Lado cultivar showed higher N, K, Ca and Mg contents than Kastilo and Romario.

Key words: Capsicum annuum; foliar fertilization; fertilizer technology; abiotic stress.




Toochukwu Ekwutosi OGBULIE, Chioma Chiemezie OKORE, Agunna Everest EJELE

Abstract. Heavy metals removal from polluted soil was comparatively assayed using a chemical surfactant (CHS/SLS), two crude biosurfactants (CB) and two biosurfactants producing isolates (BPI), Odoribacter splanchnicus DSM 20712 isolated from waste battery dumpsite soil (WBS); an unidentified bacterium clone (BC) JX981747 isolated from cassava mill soil (CSM). The biosurfactant treatments were set up in six experimental microcosms (treatments C-H) and two controls (A-B) for 16 weeks. The initial recorded values of the test heavy metals in the soil samples were 304.40 mg/kg, 131.40 mg/kg, 724.80 mg/kg and 0.38 mg/kg for Cu, Pb, Zn and Cd respectively; but it significantly (p<0.05) reduced after amendments. The test soil sample treated with composite of 60ml of crude biosurfactant from BC JX981747 and 5.2g of the producing cells (G) had the lowest Pb value of 12.40 mg/kg. Zn and Cd were also reduced more in soil treated with only CB of O. splanchnicus cells (C). The value of 232.96 mg/kg was observed in Cu concentration of soil percolated with composite of O. splanchnicus and its CB (F) indicating inefficiency in Cu removal from soil, but the use of only the CB (C) depict a contrasting ability as significant reduction in the Cu value of 52.37 mg/kg was observed. Comparatively, the CHS had negligible significant difference in metal reduction ability on soil over crude biosurfactant aside those treatment with F and G. Cation exchange ability (CEC), Organic carbon, organic matter content of the test soils all increased with the peak observed in CHS, but the use of organic surfactant is encouraged due to its environmentally friendly features. This study predicts the use of the test biological surfactants in soil conservation and management.

Key words: Bioremediation; biosurfactant; exchangeable cations; heavy metals; organic carbon; organic matter.




Nassila MEZIANI, Abderrezak CHEBOUTI, Hamenna BOUZERZOUR

Abstract. The study consists to evaluate the genetic variation of eight barley genotypes (Hordeum vulgare L.) from the breeding program of the Setif research station of the Technical Institute of Field Crops, using 11 SSR microsatellite markers. Genotypes used in this study were the subject of an agronomic evaluation and a study on their performance and their adaptation to diverse locations in Algeria. Thirty-one reproducible bands were detected of which 29 bands (93.5%) were polymorphic. The number of bands varies between 2 and 5, with an average number of 2.81 bands per primer. Polymorphic information content (PIC) values range from 0.21 to 0.71 with an average value of 0.46. The index of genetic dissimilarity between the studied genotypes varies between 0.313 and 0.818, with an average of 0.604. The greatest genetic similarity was recorded between Fouara and Rihane (0.313), while the lowest genetic similarity was observed between Dahbia and Tichedrett (0.818). SSR markers used showed a large differentiation among the different barley genotypes. This confirms the high capacity of SSR markers in genetic analysis to determine the genetic similarity of barley genotypes.

Key words: dissimilarity; barley (Hordeum vulgare L.); genetic variation; SSR markers.




Somayyeh GHANBARI HAMEDANI, Younes ASRI, Iraj MEHREGAN

Abstract. The genus Ephedra of the Ephedraceae contains more than 60 species of non-flowering seed plants (Gymnosperms) dispersed all through Asia, America, Europe, and North Africa. These Ephedra species have medicinal, ecological, and economic value. Ephedrine is an important active components of ephedra species. This study aimed to evaluate the content of Ephedrine alkaloid in 15 populations of E. major in Iran (NW to NE) covering the entire populations of E. major using the HPLC method. Our results showed the highest content of Ephedrine (EP) was from Chalous Road (CHL, N Iran; 14 mg/g) and Qorveh populations (QRV, W Iran; 13.6 mg/g). The lowest amounts of EP were detected in Ardebil (ARD, NW Iran; 0.9 mg/g) and Baraqan (BRQ; 0.2 mg/g) populations. No significant correlations were observed between the EP content and morphometric characters. The results showed that the amount of Ephedrine in populations grown in arid conditions was higher (high temperature) and negative correlation exists between the annual precipitation and EP content in different seasons (R2= -0.563. These results indicated a strong positive correlation between Ephedrine content and soil’s pH (R2=0.598), and average temperature (R2=0.554). This study determined the important environmental and edaphic factors affecting the EP content in E. major populations and can be exploited for further domestication and ecological investigations.

Key words: alkaloid; ecological factors; Ephedra; ephedrine; HPLC; Iran



Olha LOHOSHA, Yuliia VOROBEI, Tetiana USMANOVA

Abstract. In the cultivation of legumes, microbial preparations based on beneficial soil microorganisms are becoming increasingly important. Due to the mechanisms of biological fixation of molecular nitrogen and its conversion into a form accessible to plants, nodule bacteria are able to meet significantly the needs of crops in this element. The objective of our work was to carry out analytical selection of chickpea nodule bacteria and to study the effect of bacterization with new rhizobia strains on the structural parameters of chickpea yield in different soil and climatic zones of Ukraine. Inoculation of chickpea seeds of Skarb variety with a new strain of M. ciceri ND-64, which isolated from chickpea nodules of Pamiat variety, contributed to the increased number, weight of nodules and nitrogenase activity compared to the control (without inoculation), positive control (inoculation by M. cicerі Н-12) and M. cicerі ND-101, isolated from chickpea nodules of Skarb variety. It was established that the bacterization of M. ciceri ND-64 contributed to the increase of productivity and yield of chickpea plants when grown in Polissia and Steppe zone of Ukraine. Considering the obtained data, the new strain M. ciceri ND-64 is a promising bioagent of a microbial preparation for bacterization of chickpea in order to form an effective legume-rhizobial symbiosis and increase the productivity of this culture.

Key words: Mesorhizobium ciceri; chickpea; symbiosis; nitrogen-fixing activity; yield.




Khaoula MENAIAIA, Fadila KHALDI, Amina OUAHAB, Chawki BENSOUICI

Abstract. The current work concerns a medicinal plant Marrubium vulgare L. grown in Souk Ahras region (Northeastern of Algeria). Widely used by the natives of this region for their extraordinary therapeutic properties such as: antioxidant, antiinflammatory, antibacterial and analgesic activities. The aims of this study were to evaluate the phenolics, flavonoids and tannins composition, and antioxidant activities of hexane, dichloromethane, acetone and methanol extracts from leaves of Marrubium vulgare L. by using different methods. The extracts were a good source of phenolics and flavonoids. Acetonic extract from the aerial part of M. vulgare had the highest total phenolic and flavonoid contents with 171.45±3.38 µg GAE mg -1 E and 78.33±0.29 µg QE mg-1 E, respectively. While the hexane extract had the most condensed tannins content with 90±0.01 µg CE mg -1 E. The antioxidant activity showed the good antioxidant capacity of extracts (dichloromethane, acetone and methanol) in DPPH, ABTS, β-Carotene, CUPRAC, Phenanthroline and GOR assays. While the hexane extract showed the lowest antioxidant activity in DPPH, β-Carotene and GOR assay. Weak antioxidant activity in Reducing power was observed for all extracts with >200 µg/mL. On the other hand, these extracts showed no activity in Ferrous ions chelating assay, exceptionally the methanol extract which showed a very weak activity >800 µg/mL. The results showed that the concentrations of TPC, TFC and CTC were not significantly different (p > 0.05). However, a highly significant difference in all antioxidant activities was observed (p < 0.001) except Phenanthroline activity which presented a non-significant difference (p > 0.05). We have also tested the effect of the different extracts against two phytopathogenic fungi strains by the disc-diffusion method. The inhibitory effect were important for the two tested strains. The extracts of M. vulgare have important antifungal activity. Statistical analysis indicates no significant differences at P > 0.05. Based on These results, it is right to conclude that M. vulgare is an important source of bioactive compounds with antioxidant and antifungal properties.

Key words: Marrubium vulgare L.; phenolic compounds; antioxidant activity; flavonoids; antifungal activity.




Onyebuchi Ephraim NWANKWO, Samuel Akinniyi ODEWO, Babatunde Adeyemi AJANI, Luke Temitope SOYEWO

Abstract: Taxonomic studies were carried out on F. capensis, F. thonningii and F. umbellata based on macro-morphology and foliar epidermis to specifically state the diagnostic features of the three species used as vegetable. The young leaves of the species are very delicious vegetables. Standard methods were used for the studies. The results of the studies showed that the leaf shape was elliptic in F. thonningii and F. capensis and ovate in F. umbellata. Results of the studies showed considerable variations of leaf size among the three species studied. The leaf length of the F. capensis ranged from 7.0-11.0 cm with the mean of 10.5, the width ranges from 4.9-9.0 cm with the mean of 7.0 while the leaf length of F. thonningii ranged from 6.0-10.7 cm with the mean 9.8, leaf width ranged from 4.7-8.2 cm with the mean 5.0. The length of the F. umbellata ranged from 7.6-13.4 cm with the mean 9.0, the width ranged from 5.9-11.2 cm with the mean 8.0. The three species studied were hypostomatic as anomocytic stomata were common on the abaxial surfaces of F. capensis and F. thonningii while pericytic stomata were present in F. umbellata. Oblong-elliptic leaf shape with crenate margin, leaf apex: acute, leaf and fruit size, pentagonal epidermal cell shape are unique to F. capensis. Ficus thonningii possesses elliptic leaf shape, apiculate leaf apex and hexagonal epidermal cell shape while F. umbellata has ovate leaf shape with cordate base, cuspidate leaf apex, irregular cell shapes and multicellular trichomes on the adaxial surface. Proper identification of plant species is the first step to achieving credible and rewardable results of research on plants.

Key words: Ficus; macromorphology; foliar epidermis; vegetables; Ebonyi State; Nigeria.




Sid Ahmed SAADI, Atika MEKLAT, Salim MOKRANE, Hafsa YAICHE ACHOUR*, Michael D. HOLTZ, Hans-Peter KLENK, Noureddine BOURAS

Abstract. Actinobacteria originated from extreme ecosystems remains the most interesting source of new antibiotics. An actinobacterial strain, named AHO23, was isolated from Ahaggar region soil in Algeria, using the dilution agar plating method with chitin-vitamins B medium supplemented with nalidixic acid and actidione. The morphological characteristics showed that strain AHO23 belonged to the genus Saccharothrix. Analysis of the 16S rRNA gene sequence of this strain AHO23 showed 98.95% of similarity with the closely related species Saccharothrix xinjiangensis NBRC 101911T. AHO23 exhibited a strong activity against Gram-negative bacteria (15-36 mm), as well as good activity against Gram-positive bacteria (29-34 mm). Candida albicans (ATCC10237) was also inhibited (20 mm). Moreover, AHO23 presented a strong activity against mycotoxigenic and phytopathogenic fungi (21-36 mm), with the highest activity (36 mm) against Fusarium culmorum. Bioactive metabolites from AHO23 culture on ISP2 medium were extracted using four organics solvents: n-hexane, dichloromethane, ethyl-acetate and nbutanol. The highest antibacterial activities were detected in the n-butanol extract.

Key words: Actinobacteria; Saccharothrix; Ahaggar soil; 16S rRNA gene; antimicrobial activity.




Tamara SIRBU, Cristina MOLDOVAN, Olga TURCAN

Abstract. The assessment of the viability and stability of micromycetes stored in NCNM, as potential producers of active substances, for 15 years by different methods: subculturing, under a layer of mineral oil, lyophilized, showed that all are viable and retain their morpho-cultural characteristics according to strain passport. Some cultures kept under mineral oil Vaseline or infected during storage. The safest method of preservation for long-term storage is lyophilization. The viability of strains belonging to the genus Aspergillus after 15 years of preservation in lyophilized state varies in the limits of 71.0-87.2%; for the strains of the genus Penicillium – 65.2-86.8%, and for those of the genus Fusarium – 54.0-70.0%, in comparison with viability established immediately after lyophilization.

Key words: protective media; viability; lyophilization; subculturing; preservation under mineral oil




Slavka KALAPCHIEVA, Valentin KOSEV, Viliana VASILEVA

Abstract. Correlation dependences of quantitative traits are of importance for further breeding process in crops. Trial with 10 samples of garden pea was performed during three years period. Both phenotypic and genotypic relationships between major quantitative traits have been evaluated. For most of the quantitative indicators, the phenotypic relationships were found insignificantly lower than the values of their genetic correlation coefficients. The next correlation coefficients were found with significant genotype dependencies: between the plant height and internode length (r = 0.936); height to first fertile node and average number of grains per pod (r = 0.826); 2 pods per fruiting handle with pod weight per plant (r= 0.717); number of tillers with average number of grains per pod (r= 0.709); total number of pods per plant with 2 pods per fruiting handle (r= 0.981). Strong to average was found the dependence on the green grains weight with the pods weight (r = 0.942), % of unfilled grains (r = 0.813). The described correlations between the quantitative traits showеd the prospects for their use in the breeding process, depending on the tasks given.

Key words: garden pea; correlation; trait; interrelations




Asmaa BENAISSA, Réda DJEBBAR, Lilya BOUCELHA, Aida BASSEDDIK

Abstract. This study aims at assaying the antioxidant activities of Searsia tripartita (Ucria) Moffet growing in Ahaggar (Algeria) as a continuation of our previous work on the ecophysiology characteristic of this shrub. The enzymatic antioxidant activities of catalase (CAT) and Ascorbate peroxydase (APX) were evaluated. The total phenolic contents of this plant were measured by the Folin–Ciocalteu method and the radical scavenging activity was determined by using DPPH assay (2,2 Diphenyl 1- Picryl Hydrazyl). The shrub was found to have a high antioxidant capacities and could be potential rich sources of natural antioxidants. Then, the antiradical activity (DPPH scavenging) IC50 was 153.69±0.428 µg antioxidant.mL while phenolic compound values range from 23.923 to 52.926 mg GAE·g-1 DW. Moreover, high values of antioxidant enzyme activities were recorded respectively for catalase and ascorbate peroxidase: 136.59 ± 3.412 and 135.03 ± 5.712 µmol·min-1·mg-1 proteins. Our experiments indicated that this shrub is well adapted to the arid climate of the Ahaggar region by facing the oxidative stress through a high production of antioxidants. In conclusion, the species Searsia tripartita seems to adopt a drought-tolerance type strategy towards aridity oxidative stress.

Key words: Searsia tripartita; Ahaggar; ardity; phenolic compounds; antioxidant activities.




Mounir KHERROUBI, Izzeddine Zakarya ZERROUK, Bilal RAHMOUNE, Sabri Ala Eddine ZAIDAT, Oussama MESSADI, Fazia MOUHOUCHE

Abstract. Loses caused by Callosobruchus maculatus in stored legumes are significant. In this study we were carried out in order to determine the insecticidal potential of Artemisia herba-alba, Juniperus oxycedrus L. and Pelargonium graveolens essential oils. Environmental and health concerns of synthetic insecticides highlight the need for new strategies to protect stored grains insect infestation. The contact effect of the three essential oils against adults of Callosobruchus maculatus (Coleoptera: Bruchidae) was investigated. While the oils of P. graveolens and J. oxycedrus essential oils showed the same strong insecticidal activity against C. maculatus (LD50 = 2.270% and 8.017%, respectively), the oil of A. herba-alba revealed poor activity against the insect (LD50 = 11.670%). The components of the essential oils were obtained and identified. Chromatographic analysis (GC-MS) of A. herba-alba essential oil demonstrated that chrysanthenone (31.40%) is the main constituent. And as for the J. oxycedrus L. the highest components were Alpha-pinene (64.80%). And the main components of the P. graveolens essential oil were determined as citronellol (36.00%).

Key words: Callosobruchus maculatus; biocontrol; essential oil.




Radka BOZHINOVA

Abstract. This research was carried out to assess the effect of long-term fertilization with different N rates on the concentration of Fe, Mn, Zn, Cu, Pb, Ni and Cd in soil and in oriental tobacco plants. The long-term fertilizer experiment was initiated in 1966 in Markovo, Bulgaria on rendzina soil (Rendzic Leptosols). For the purposes of the study, three N rates (0, 50, and 100 kg N·ha-1) were selected. Each treatment had three replicates with a completely randomized block design. Results showed that long-term N fertilization caused a slight decrease in soil pH compared to the unfertilized control and thus did not substantially influence trace elements’ availability. N-fertilizer rate had no significant effect on the available Fe, Mn, Zn, Cu, Pb and Cd content in the soil. Plant-available Ni content significantly increased in plots with long-term use of nitrogen fertilizer. Nitrogen fertilizer application greatly increased tobacco yield and N content in the leaves. With the increase of N fertilization rate the average concentration of the nitrogen in leaves increased from 1.35% to 2.79%. With the exception of the iron content in the upper leaves, increasing levels of N fertilization had no significant effect on Mn, Zn, Cu, Pb, Ni and Cd concentrations in oriental tobacco. Therefore, long-term application of different rates of nitrogen fertilizer had no obvious effect on the accumulation of available Fe, Mn, Zn, Cu, Pb, and Cd in soil and concentrations of trace elements in tobacco grown on alkaline soil.

Key words: nitrogen rate; trace elements; tobacco; soil.




Fatma CHEHRIT-HACID, Rabéa GACEB-TERRAK, Henia DAOUDI, Lynda HANNACHI, Boussad METNA, Abderrahmane MATI, Ferid LIMAM, Farida MATI-MOULTI

Abstract. Pistacia lentiscus, evergreen sclerophyll shrub and P. atlantica, deciduous species, belong to Anacardiaceae family. They were chosen in this study because they suit the Mediterranean climate characterized by hot and dry summers and mild winters with heavy rain. Polyphenols are involved in the response to biotic and abiotic stress. However, very few studies have been conducted on the polyphenols of P. lentiscus and P. atlantica and to our knowledge, there are no studies on phenolic variability. The aim of this work is to study polyphenolic variability in Pistacia lentiscus and P. atlantica leaves, in relation to environmental factors. The environmental factors taken into account are altitude, slope exposure and some soil characteristics as pH, organic matter and Calcium carbonate. The total polyphenols were extracted with 70% ethanolic solvent and phenolic aglycones with diethylether after acid hydrolysis. HPLC-DAD was used to identify and quantify polyphenolic compounds in the extracts when GC-MS was used to identify the aglycones. Total polyphenols, total flavonoids and hydrolysable tannins were quantified using an UV/Visible spectrophotometer. In order to understand the variability within and between the two species and its relation to certain environmental factors, principal compound analysis was performed. The results obtained showed the presence of high amounts of polyphenols in the leaves of both species, the total polyphenol content varies from 19.50 ± 2.5 mg‧g-1 DM to 28.56 ± 3.61 mg‧g-1 DM. They also show intra and inter specific variability with respect to the environmental factors. Presence of several chemotypes of the two species were suggested. P. atlantica was qualitatively and quantitatively richer and relatively more homogeneous than P. lentiscus wich presents high intraspecific variability, fourteen compounds were detected by HPLC-DAD and more than twenty five by CG-MS.

Key words: Pistacia lentiscus; P. atlantica; phenolic compounds; environmental factors




Cristina STANCĂ-MOISE

Abstract. The Romanian Lepidopterological Society nominated the species Zerynthia polyxena as the insect of the year 2021. This is the reason why the present paper is dedicated to this species. The present paper has as a purpose to publish the data of collecting of the Zerynthia polyxena and Zerynthia cerisyi ferdinandi in the following Lepidoptera collections, preserved at the Natural History Museum in Sibiu, Romania: Daniel Czekelius, Eugen Worell, Viktor Weindel, Heinrich Hann von Hannenheim, Rolf Weirauch and Eckbert Schneider. Alltogether 82 specimens of the Zerynthia polyxena and 17 specimens of the Zerynthia cerisyi ferdinandi were found. The most numerous are specimens from Transylvania and Banat, but they are also samples collected from the other historical regions of Romania (Crishana, Dobrudja, Maramures, Moldova, Muntenia and Oltenia). In the studied collections there are also specimens originating from other European countries: Austria, Serbia, North Bulgaria and North Macedonia.

Key words: Zerynthia polyxena; Zerynthia cerisyi ferdinandi (Lepidoptera, Papilionidae)